| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.180891 |
| Chromosome: | chromosome_11 |
| Location: | 2467642 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre11.g475600 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCGCTCGCTTTCGCCACCTGTGCGGCAGAA |
| Internal bar code: | TGTCCAGGTGTTGTGCGAAACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 452 |
| LEAP-Seq percent confirming: | 95.7105 |
| LEAP-Seq n confirming: | 1785 |
| LEAP-Seq n nonconfirming: | 80 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAATCAAGGAGCACAGACA |
| Suggested primer 2: | TCCTTTTGTCTGTCGTTCCC |