| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.180900 |
| Chromosome: | chromosome_6 |
| Location: | 3256812 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g276700 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGGAGCAGGAGTTGTAAGGGTGAGGGCCTG |
| Internal bar code: | ATATGGGGTTTGATGCCGCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 646 |
| LEAP-Seq percent confirming: | 95.0719 |
| LEAP-Seq n confirming: | 4167 |
| LEAP-Seq n nonconfirming: | 216 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTCCCAGCTCACTCTTTG |
| Suggested primer 2: | ACACACACACACACTCCGCT |