| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.180900 |
| Chromosome: | chromosome_9 |
| Location: | 4476927 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g395769 | FAP177 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CAGCCAACGGTATATAACATGCACAAGAAG |
| Internal bar code: | CTATCGCGGGCTATATTCGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 461 |
| LEAP-Seq percent confirming: | 92.7536 |
| LEAP-Seq n confirming: | 2048 |
| LEAP-Seq n nonconfirming: | 160 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCCTTCTCCTCCTGCTTT |
| Suggested primer 2: | ACAAACCGACCACAAAGGAG |