| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.180902 |
| Chromosome: | chromosome_13 |
| Location: | 5095283 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g607300 | MAPK5 | Mitogen-Activated Protein Kinase | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CAGTCACGGAGGTGAAGACCGCCAGCGCCT |
| Internal bar code: | TCCAACCCGTGATCGTGAAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1221 |
| LEAP-Seq percent confirming: | 99.3706 |
| LEAP-Seq n confirming: | 2842 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTGTACAGCACATGATCC |
| Suggested primer 2: | ACTGCCGCACTAGCTGTACC |