| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.180915 |
| Chromosome: | chromosome 11 |
| Location: | 1087823 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467676 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCAACCGCACCACCTTCATAACCAACAAC |
| Internal bar code: | ATCGGTGATATGCCAGGAGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 45 |
| LEAP-Seq percent confirming: | 28.3186 |
| LEAP-Seq n confirming: | 64 |
| LEAP-Seq n nonconfirming: | 162 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGCCGATTATATTCCCAG |
| Suggested primer 2: | GACACCCTCGCAGACTTCTC |