Insertion junction: LMJ.RY0402.180922_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.180922
Chromosome:chromosome 17
Location:4169023
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g729950 SRX1,SRX2 (1 of 2) 1.8.98.2 - Sulfiredoxin / Peroxiredoxin-(S-hydroxy-S-oxocysteine) reductase; Related to sulfiredoxin 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATCAAGGCAGGAGGCAGGGTGCGTGTCGC
Internal bar code:ATAGTGCCGAACGTGGCCGCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:774
LEAP-Seq percent confirming:99.6662
LEAP-Seq n confirming:14034
LEAP-Seq n nonconfirming:47
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGAAACAGGGGTGAAACT
Suggested primer 2:CAAGCTGCTTTGGTAGAGGG