| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.180943 |
| Chromosome: | chromosome 7 |
| Location: | 5081893 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g347600 | (1 of 1) PTHR11059:SF0 - DNA REPAIR PROTEIN RECN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGACGTGGTGGCGGCCGCCGCCATGAAC |
| Internal bar code: | TGTTTGGTCCTGTCCAGTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1100 |
| LEAP-Seq percent confirming: | 99.6571 |
| LEAP-Seq n confirming: | 11915 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTGGGGGTGAGAATGTGT |
| Suggested primer 2: | AGCGCTGCTACTCCTTCATC |