| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.180957 |
| Chromosome: | chromosome 17 |
| Location: | 3200787 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g722100 | SSA3 | cilia-sensing, structure and/or assembly; (1 of 1) PTHR12771:SF2 - ELMO DOMAIN-CONTAINING PROTEIN 3 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAAAACATTCACGTCTTATTGTTACCGCC |
| Internal bar code: | CTGCGGCGTCTAGATCGAATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 453 |
| LEAP-Seq percent confirming: | 99.4882 |
| LEAP-Seq n confirming: | 972 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGCCTTCCGTTCCTTATC |
| Suggested primer 2: | GATCAGGAGGATGTGGGAGA |