| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.180968 |
| Chromosome: | chromosome 11 |
| Location: | 383978 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467578 | (1 of 1) K02903 - large subunit ribosomal protein L28e (RP-L28e, RPL28) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGACGCTTCGCCCACGTTTCTGACCCGGC |
| Internal bar code: | TACGGAGGCCAACCCGGACGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1005 |
| LEAP-Seq percent confirming: | 99.6465 |
| LEAP-Seq n confirming: | 15220 |
| LEAP-Seq n nonconfirming: | 54 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATGATTTCCGGATTGAGC |
| Suggested primer 2: | TGACCATGAAGAAGCACAGC |