| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.180976 |
| Chromosome: | chromosome 6 |
| Location: | 5395895 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g283850 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGTGTTTGGGCCTAGTGACGTCCGGTAAC |
| Internal bar code: | GACGGATAGTTTACGGAGCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 609 |
| LEAP-Seq percent confirming: | 75.2168 |
| LEAP-Seq n confirming: | 1041 |
| LEAP-Seq n nonconfirming: | 343 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTATACCTATCGCCCCGC |
| Suggested primer 2: | TGGAAGGGTGTCACACAGAA |