| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.180989 |
| Chromosome: | chromosome 8 |
| Location: | 2324427 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g370550 | (1 of 1) 1.1.99.39 - D-2-hydroxyglutarate dehydrogenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTGACAGGTGATGACCAATGGGTCATTC |
| Internal bar code: | TGTAGAATGGCTCGTGCAAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 563 |
| LEAP-Seq percent confirming: | 99.5849 |
| LEAP-Seq n confirming: | 14393 |
| LEAP-Seq n nonconfirming: | 60 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCAGAGAACGCTGTGAAT |
| Suggested primer 2: | CGGATGAGCTGTGTAGACGA |