| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.181060 |
| Chromosome: | chromosome 15 |
| Location: | 1091082 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g638304 | NCL30,OPR74 | Nuclear Control of chloroplast Like 30 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTTTGGCGGGGCACCTACCGTGCTGGTG |
| Internal bar code: | CAAGGTTCTGGTCGGGTGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 216 |
| LEAP-Seq percent confirming: | 97.4563 |
| LEAP-Seq n confirming: | 1226 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCCGTCCGCATCAATTAC |
| Suggested primer 2: | TGTAGGCAGTGAGCAGGATG |