| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.181066 |
| Chromosome: | chromosome 1 |
| Location: | 595934 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g003475 | (1 of 2) 3.4.24.35 - Gelatinase B / Type V collagenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTTCAGGCCGTGTGTGCGCGTGCCGCAG |
| Internal bar code: | GGTGCGGTCGAAGGAACGCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 310 |
| LEAP-Seq percent confirming: | 99.8971 |
| LEAP-Seq n confirming: | 1941 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTATTGGCACTGCAACCA |
| Suggested primer 2: | CTCTCCCTCTCCCTCTCCCT |