| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.181068 |
| Chromosome: | chromosome 6 |
| Location: | 2222110 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g266276 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATGGGCTCGGTCGCATGGCTCCGCATTG |
| Internal bar code: | GAGGATCCCGGTGTAAAGCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1164 |
| LEAP-Seq percent confirming: | 97.3998 |
| LEAP-Seq n confirming: | 16819 |
| LEAP-Seq n nonconfirming: | 449 |
| LEAP-Seq n unique pos: | 87 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGCCTAGCGGAATGAGTC |
| Suggested primer 2: | TGAAGCCAAGAACTGCATTG |