Insertion junction: LMJ.RY0402.181074_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.181074
Chromosome:chromosome 11
Location:1103722
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g467678 COP6,HKR2 (1 of 5) IPR001660//IPR013761 - Sterile alpha motif domain // Sterile alpha motif/pointed domain; Histidine cyclase rhodopsin 2 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGCCCACCGTCCGGACAAACACGCGGGTT
Internal bar code:AGAAACCTAGGCGCTGAACAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:509
LEAP-Seq percent confirming:98.8562
LEAP-Seq n confirming:1210
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTGCGTGTGTGTGTGTTT
Suggested primer 2:TGGTGAGGTGGACCTGTGTA