| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.181077 |
| Chromosome: | chromosome 2 |
| Location: | 1393711 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g083350 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAATCAGTATCCGCGCGTTGCTTTGGATA |
| Internal bar code: | CTTTGAGGCCGTCACGAGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1016 |
| LEAP-Seq percent confirming: | 97.6863 |
| LEAP-Seq n confirming: | 11864 |
| LEAP-Seq n nonconfirming: | 281 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTGGTGTTGCCCTTCATA |
| Suggested primer 2: | TACCAGCCCAAACTAAACCG |