| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.181100 |
| Chromosome: | chromosome 14 |
| Location: | 3182224 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g629050 | (1 of 1) PF02298 - Plastocyanin-like domain (Cu_bind_like) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGCAACGGGTGCGGCCTAGCGGAAGGTG |
| Internal bar code: | TTGGCGAGCGCCGTATAACAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1144 |
| LEAP-Seq percent confirming: | 99.4415 |
| LEAP-Seq n confirming: | 7656 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCCTCCTTGTAGCACTCA |
| Suggested primer 2: | GGTCTCGCTTTGCCTGATAG |