| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.181147 |
| Chromosome: | chromosome_2 |
| Location: | 7902290 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g141400 | PCK1 | Phosphoenolpyruvate carboxykinase | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCCTCCCTGACTCGTCCGTGTAGCCAACCT |
| Internal bar code: | CATACGAGGATTGACTTAGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 331 |
| LEAP-Seq percent confirming: | 99.6119 |
| LEAP-Seq n confirming: | 6674 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACGACAACACACGCTCTC |
| Suggested primer 2: | TGTCCGGGAGATGAGGATAG |