| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.181172 |
| Chromosome: | chromosome 13 |
| Location: | 2621093 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g581350 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGCGACCCGGCCGAGCCGCCCGCGGCCA |
| Internal bar code: | ACGTCATCGTGTACACTTGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 348 |
| LEAP-Seq percent confirming: | 50.6667 |
| LEAP-Seq n confirming: | 76 |
| LEAP-Seq n nonconfirming: | 74 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGAACGGTGCTTACTGCTT |
| Suggested primer 2: | CGAGCTTCTTTGCCATTAGG |