| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.181193 |
| Chromosome: | chromosome 8 |
| Location: | 1460409 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g364550 | (1 of 3) K10587 - ubiquitin-protein ligase E3 A (UBE3A, E6AP) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCTCATCTGGCTGCGGGTGCGTTGCTAC |
| Internal bar code: | TTCGTGAGGTTCTTGAACATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 306 |
| LEAP-Seq percent confirming: | 99.6616 |
| LEAP-Seq n confirming: | 17672 |
| LEAP-Seq n nonconfirming: | 60 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGCTTTCCAATGACGCTC |
| Suggested primer 2: | TCGATGCTTGCTTTGTGTTC |