| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.181222 |
| Chromosome: | chromosome 16 |
| Location: | 3724524 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g688950 | (1 of 8) 2.4.1.5 - Dextransucrase / Sucrose 6-glucosyltransferase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGATGGCGGCTAACAGCCAGTCGCGCGCA |
| Internal bar code: | GCCGAAAGTGGCCAGGGGTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 635 |
| LEAP-Seq percent confirming: | 98.7547 |
| LEAP-Seq n confirming: | 4758 |
| LEAP-Seq n nonconfirming: | 60 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTGCCGTACATCCCAGTA |
| Suggested primer 2: | AATGCCAAACGTCCTACCTG |