| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.181250 |
| Chromosome: | chromosome 6 |
| Location: | 5313733 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g283050 | LHCA1 | Light-harvesting protein of photosystem I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGCCACCAACGGCATCTCCGTCCCCTTC |
| Internal bar code: | GGTGATGAACCTTACGATTAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 696 |
| LEAP-Seq percent confirming: | 99.629 |
| LEAP-Seq n confirming: | 1880 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAACCCGCTTCTACCCCTT |
| Suggested primer 2: | AGTTGTTAACACGCACGCAG |