| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.181255 |
| Chromosome: | chromosome 7 |
| Location: | 745796 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g317800 | (1 of 1) PF04091 - Exocyst complex subunit Sec15-like (Sec15) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGTACACCCCGCCGCCCAAACTCGCCAC |
| Internal bar code: | CGAGTAGGTCCTTCCTGCTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 100 |
| LEAP-Seq percent confirming: | 77.7778 |
| LEAP-Seq n confirming: | 119 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACAAGGCGTATGTGTGTG |
| Suggested primer 2: | GCAACTAAAATGAGCGGAGC |