| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.181258 |
| Chromosome: | chromosome 6 |
| Location: | 8062198 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g304450 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGTCGCTGATCCTTCCAGCTAGGATCCG |
| Internal bar code: | GTAGATTGCCGTGACTGAAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 357 |
| LEAP-Seq percent confirming: | 99.729 |
| LEAP-Seq n confirming: | 368 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAAACTATCGGCGCAGAGC |
| Suggested primer 2: | CGAAACCTCATCCGTTCATT |