| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.181272 |
| Chromosome: | chromosome 12 |
| Location: | 2666295 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g504150 | NFU2,NIFU2 | Iron-sulfur cluster assembly protein; (1 of 1) PTHR11178//PTHR11178:SF1 - IRON-SULFUR CLUSTER SCAFFOLD PROTEIN NFU-RELATED // NFU1 IRON-SULFUR CLUSTER SCAFFOLD HOMOLOG, MITOCHONDRIAL | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACCCGCGCAACATGAACACCAGGGATGC |
| Internal bar code: | GGTCGCGATCACGGGCCGTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 766 |
| LEAP-Seq percent confirming: | 99.8532 |
| LEAP-Seq n confirming: | 3402 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAAACGGTGTTTCGGAAGG |
| Suggested primer 2: | GGACATTGTGTTCAAGGGCT |