| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.181272 |
| Chromosome: | chromosome 12 |
| Location: | 3507345 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g512950 | CIT1 | (1 of 1) K07240 - chromate transporter (chrA); Chromate ion transporter | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACACGGCTCATAAACCTTCCTCGGCGTTG |
| Internal bar code: | GATTCGCTAGAGTTGAGGTACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1045 |
| LEAP-Seq percent confirming: | 75.7535 |
| LEAP-Seq n confirming: | 1131 |
| LEAP-Seq n nonconfirming: | 362 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCAACTTCAGCGCCTACCT |
| Suggested primer 2: | AACAAACCAAGACCCGAGTG |