| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.181278 |
| Chromosome: | chromosome 13 |
| Location: | 1901202 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g575900 | CGL117 | (1 of 1) PF04959//PF12066 - Arsenite-resistance protein 2 (ARS2) // Domain of unknown function (DUF3546) (DUF3546); predicted protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGGCTGCGACCACGGTTGTATCGATCAC |
| Internal bar code: | ACCGCGTTAATACCCCCCTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 298 |
| LEAP-Seq percent confirming: | 95.124 |
| LEAP-Seq n confirming: | 2302 |
| LEAP-Seq n nonconfirming: | 118 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACTCCTGGAACATGCGGT |
| Suggested primer 2: | AAAGCTAAAGCCAGGAAGGC |