| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.181287 |
| Chromosome: | chromosome 12 |
| Location: | 7568041 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g555450 | PMK,IPK,IMPK | (1 of 1) 2.7.1.140 - Inositol-tetrakisphosphate 5-kinase / 1D-myo-inositol-tetrakisphosphate 5-kinase; inositol-tetrakisphosphate 5-kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAACTTGCGAAAGGGTTCAGTGGCAACGG |
| Internal bar code: | TATGGATTGCGGAAGCCCACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1146 |
| LEAP-Seq percent confirming: | 99.7287 |
| LEAP-Seq n confirming: | 8086 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 67 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTCATGTGCTGCGTTCAG |
| Suggested primer 2: | CTGAGGATGTGGCTGCTGTA |