| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.181336 |
| Chromosome: | chromosome 3 |
| Location: | 7497071 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g205450 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTGTGGATGTGCGTTAGAAATGCACAAA |
| Internal bar code: | TCGTCTGGATTGTGTGCGTCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 360 |
| LEAP-Seq percent confirming: | 99.5606 |
| LEAP-Seq n confirming: | 3172 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGTGTACGACCCTGCAAC |
| Suggested primer 2: | AATGTTGCGCAAAGACAGTG |