| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.181341 |
| Chromosome: | chromosome 1 |
| Location: | 4204222 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g027950 | IFT74,CMG1,IFT71,IFT72 | (1 of 1) PTHR31432:SF0 - INTRAFLAGELLAR TRANSPORT PROTEIN 74 HOMOLOG; Intraflagellar transport protein 74/72 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGAAGGCCGGGCGAAGTCAACCTCGAACT |
| Internal bar code: | GACGGAGCCGCGGGAACCTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 635 |
| LEAP-Seq percent confirming: | 97.561 |
| LEAP-Seq n confirming: | 1520 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCTAATGTGCTAAGGAGC |
| Suggested primer 2: | CCTGCCTCACCTTCATGTTT |