Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.181342 |
Chromosome: | chromosome 10 |
Location: | 1734986 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre10.g430650 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGGAAGTTTCTCAGCTGCAAATCCGTGC |
Internal bar code: | GACAACGAACGATGGTGAAAGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 897 |
LEAP-Seq percent confirming: | 82.36 |
LEAP-Seq n confirming: | 2750 |
LEAP-Seq n nonconfirming: | 589 |
LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGTCTTTCTTTTGACGAGCC |
Suggested primer 2: | GACTCAGTGCATTGCTGCAT |