Insertion junction: LMJ.RY0402.181422_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.181422
Chromosome:chromosome 4
Location:1526531
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g213751 (1 of 43) IPR011989 - Armadillo-like helical 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCGCCCTGTATCCGCTTTCCGTCGCTGGT
Internal bar code:GCCTTACGGGCGAAGGGGGATT

Confirmation - LEAP-Seq

LEAP-Seq distance:645
LEAP-Seq percent confirming:99.0521
LEAP-Seq n confirming:836
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:TACCAGCCCAAACTAAACCG
Suggested primer 2:AGTATTTGGCCTGTGGATCG