| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.181459 |
| Chromosome: | chromosome 12 |
| Location: | 9685134 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g556228 | NDA7 | Type-II NADH dehydrogenase; (1 of 5) 1.6.5.9 - NADH:ubiquinone reductase (non-electrogenic) / Ubiquinone reductase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGTGTCATGGGAAACCTTGTTCGTAAGC |
| Internal bar code: | GCGGACCGAAGGGACCCAGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1120 |
| LEAP-Seq percent confirming: | 98.5942 |
| LEAP-Seq n confirming: | 34366 |
| LEAP-Seq n nonconfirming: | 490 |
| LEAP-Seq n unique pos: | 132 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGAACCTTGTGCTCTCTT |
| Suggested primer 2: | CACTCGGCATAGCTGTTTGA |