| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.181479 |
| Chromosome: | chromosome 14 |
| Location: | 4029377 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g633500 | (1 of 1) IPR024224 - DENND6 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGAGGTGCTGCGCTGGCCGCCCCCAGTG |
| Internal bar code: | CGCCTGCGAAGTATTTGAAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 184 |
| LEAP-Seq percent confirming: | 87.1795 |
| LEAP-Seq n confirming: | 136 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCAATACCCACAACCAGC |
| Suggested primer 2: | CTTCTTCTTCCGCATCAAGC |