| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.181529 |
| Chromosome: | chromosome_13 |
| Location: | 1618857 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g573500 | FAP95 | Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGGTGTATGTGTGTGCAGGTTTTGAGGGTA |
| Internal bar code: | TTCATGAACACTCGGCTTCCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 582 |
| LEAP-Seq percent confirming: | 99.3677 |
| LEAP-Seq n confirming: | 7858 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCTATGCTTGGCGTTGAG |
| Suggested primer 2: | TGCAGATGAGGGTGAGAGTG |