| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.181538 |
| Chromosome: | chromosome 17 |
| Location: | 4448702 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g732150 | FAP130b,FAP130 | Flagellar Associated Protein 130 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGGAATGAGTGTCGAGAATGGGAGTTGA |
| Internal bar code: | GAATTCTCGCTGTACGGTTACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 261 |
| LEAP-Seq percent confirming: | 75.6645 |
| LEAP-Seq n confirming: | 1281 |
| LEAP-Seq n nonconfirming: | 412 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGAAAGGAGCGTCTCAAA |
| Suggested primer 2: | TTAGTCTTAGAGGCGGGCAA |