Insertion junction: LMJ.RY0402.181551_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.181551
Chromosome:chromosome 11
Location:877332
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g467653 (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGGTACGTCGCCGTACGGCACACGGGTCA
Internal bar code:CCGACACGCTCCACATTGCGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:478
LEAP-Seq percent confirming:88.9226
LEAP-Seq n confirming:586
LEAP-Seq n nonconfirming:73
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:ACCAGACCAACGTCTTCACC
Suggested primer 2:CGAAACGAACAGATGAAGCA