| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.181560 |
| Chromosome: | chromosome 16 |
| Location: | 4189234 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g668750 | MTA5 | Hypothetical protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGCTTTGGTTACACCTATGAGGACGACG |
| Internal bar code: | ACCACGGACGGGCGCTTGCCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 695 |
| LEAP-Seq percent confirming: | 99.7953 |
| LEAP-Seq n confirming: | 4387 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTCACTTGCACATCCACC |
| Suggested primer 2: | ACGCATGTAAAGGCAGGAAG |