| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.181576 |
| Chromosome: | chromosome 7 |
| Location: | 875444 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g318651 | AOF4 | (1 of 1) 1.5.3.16 - Spermine oxidase / SMO; Flavin-containing amine oxidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGAGGGATAGGGATAGGGAGAGGGAGGG |
| Internal bar code: | GAAACGTGCCGGTCACGGTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 150 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 255 |
| LEAP-Seq n nonconfirming: | 51 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACACGAGTTATGCCCACA |
| Suggested primer 2: | AATATGTTCCTCAGCACGGC |