| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.181610 |
| Chromosome: | chromosome 12 |
| Location: | 8591228 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g547300 | (1 of 14) 3.6.3.25 - Sulfate-transporting ATPase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGTTGGGCAGCCCCGACCCGCCCCCGCC |
| Internal bar code: | GAGCCACAGATAGGTGGGCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 894 |
| LEAP-Seq percent confirming: | 99.9084 |
| LEAP-Seq n confirming: | 4365 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAACTACCCACCCAACATC |
| Suggested primer 2: | TGCGGTGGGGTAGTCTAATC |