| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.181611 |
| Chromosome: | chromosome 5 |
| Location: | 1970897 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241639 | (1 of 3) K09858 - SEC-C motif domain protein (K09858) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTACCACGTCCTCCCCCTTCCACCACGTCC |
| Internal bar code: | CAGAGAGCGCGTTGCGATCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 314 |
| LEAP-Seq percent confirming: | 99.6904 |
| LEAP-Seq n confirming: | 322 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACATTCGCGACGTGAAGA |
| Suggested primer 2: | ACCTCATCTCATCACAGCCC |