| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.181628 |
| Chromosome: | chromosome_9 |
| Location: | 3677219 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g390763 | Small Rab-related GTPase | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTGCAGGGGTGATGGGCTGGGTTTCTAGGA |
| Internal bar code: | CGAGTAACAAAGCGGAATCTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2 |
| LEAP-Seq percent confirming: | 89.1204 |
| LEAP-Seq n confirming: | 770 |
| LEAP-Seq n nonconfirming: | 94 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCTCGTGTAGCTTGAGGC |
| Suggested primer 2: | ATATTTGCCTCACTCACCCG |