Insertion junction: LMJ.RY0402.181657_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.181657
Chromosome:chromosome 16
Location:2076090
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g657450 MPA12 Metallophosphoesterase/metallo-dependent phosphatase; (1 of 1) 3.6.1.13//3.6.1.16//3.6.1.53 - ADP-ribose diphosphatase / ADPR-PPase // CDP-glycerol diphosphatase / CDP-glycerol pyrophosphatase // Mn(2+)-dependent ADP-ribose/CDP-alcohol diphosphatase / Mn(2+)-dependent ADP-ribose/CDP-alcohol pyrophosphatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAAGATGCAATGCGGTGGGGCACTGTCCGC
Internal bar code:GGTATGAATAGTCAACCCATAA

Confirmation - LEAP-Seq

LEAP-Seq distance:139
LEAP-Seq percent confirming:92.3077
LEAP-Seq n confirming:36
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTTCCTGGGAGACGTGAT
Suggested primer 2:GCTTACGGCAGGTTCTGAAG