| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.181658 |
| Chromosome: | chromosome_17 |
| Location: | 1622100 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g708000 | FAP260 | Flagellar Associated Protein, membrane-associated | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGTAGCCGGCGGTGACGGAAGCGGGGCCGT |
| Internal bar code: | CACCTAGCTGTTAAGGTTGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 683 |
| LEAP-Seq percent confirming: | 99.7685 |
| LEAP-Seq n confirming: | 5603 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATGAAGTTGCTTTCGTCG |
| Suggested primer 2: | GCCAGTTGGACGGTGATAGT |