| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.181660 |
| Chromosome: | chromosome 13 |
| Location: | 3338141 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g586650 | FXL1 | FixL-like PAS domain protein; (1 of 12) 2.7.13.3 - Histidine kinase / Protein kinase (histidine) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCTTCCGAGTCGGGCAGCAGCATGGGCG |
| Internal bar code: | GCGAGTAGCAGTTAATATACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 504 |
| LEAP-Seq percent confirming: | 99.1736 |
| LEAP-Seq n confirming: | 360 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGTACACGATGATGAGGG |
| Suggested primer 2: | ACGCTGATCGTCAGTGAGTG |