| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.181708 |
| Chromosome: | chromosome 13 |
| Location: | 4740152 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g604850 | (1 of 1) IPR026126 - BRISC and BRCA1-A complex member 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTACTTGATGAGTTATAGTGCCAAGCAAG |
| Internal bar code: | ACAAAGTCCGGTCCTAGTCCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 946 |
| LEAP-Seq percent confirming: | 95.8477 |
| LEAP-Seq n confirming: | 554 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTTTGCAGGCACAGTAGG |
| Suggested primer 2: | ACTTTACGCTGCCAATACCG |