| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.181711 |
| Chromosome: | chromosome 14 |
| Location: | 1289774 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g616650 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCGCACAAGCAGCCAGGTTACAGCTCAG |
| Internal bar code: | TAATGTCTGTCTGTATACTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 707 |
| LEAP-Seq percent confirming: | 91.958 |
| LEAP-Seq n confirming: | 526 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGTTATTTGGGTCGTCGT |
| Suggested primer 2: | CTCTTCGCTCCTGTTGGTTC |