Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.181727 |
Chromosome: | chromosome 3 |
Location: | 4298514 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g174672 | (1 of 1) 3.1.1.93 - Mycophenolic acid acyl-glucuronide esterase / Mycophenolic acid acyl-glucuronide deglucuronidase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAGCACGTACCCGTGCGTGCGCAAGGCC |
Internal bar code: | AACTCGTAGGCCGCACGTTGCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 831 |
LEAP-Seq percent confirming: | 78.1109 |
LEAP-Seq n confirming: | 3986 |
LEAP-Seq n nonconfirming: | 1117 |
LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CACCTAAACCTTCCGTGCAT |
Suggested primer 2: | GTCTGCTTCGACTACTCGGG |