| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.181728 |
| Chromosome: | scaffold 24 |
| Location: | 60366 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre24.g755547 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGTGGGCCGGGATTGTAAGATTGTAGCC |
| Internal bar code: | TCGGTAGGATAGTCTCGGCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 945 |
| LEAP-Seq percent confirming: | 99.5462 |
| LEAP-Seq n confirming: | 4826 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGGTTGACAGGATGGCAC |
| Suggested primer 2: | TGGGTCCCTATCACATCCAT |