| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.181732 |
| Chromosome: | chromosome 2 |
| Location: | 1029365 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g080550 | PDE26 | (1 of 1) PF00012//PF00233 - Hsp70 protein (HSP70) // 3'5'-cyclic nucleotide phosphodiesterase (PDEase_I); 3'%252C5'-cyclic-nucleotide phosphodiesterase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTAAGTGGTGAGAGGCAGCACGGGATGTT |
| Internal bar code: | GATGTCACGTAAATAATCTCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 393 |
| LEAP-Seq percent confirming: | 99.3792 |
| LEAP-Seq n confirming: | 21613 |
| LEAP-Seq n nonconfirming: | 135 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAAACAACCACCAGCTCCG |
| Suggested primer 2: | AATGGCTTGGCTAACACACC |